diff --git a/packages/nodes-base/nodes/Aws/S3/GenericFunctions.ts b/packages/nodes-base/nodes/Aws/S3/GenericFunctions.ts
index 92d620d102..fd7fe838f6 100644
--- a/packages/nodes-base/nodes/Aws/S3/GenericFunctions.ts
+++ b/packages/nodes-base/nodes/Aws/S3/GenericFunctions.ts
@@ -36,7 +36,7 @@ export async function awsApiRequest(this: IHookFunctions | IExecuteFunctions | I
 	// Sign AWS API request with the user credentials
 	const signOpts = {headers: headers || {}, host: endpoint, method, path: `${path}?${queryToString(query).replace(/\+/g, '%2B')}`, body};
 
-	sign(signOpts, { accessKeyId: `${credentials.accessKeyId}`, secretAccessKey: `${credentials.secretAccessKey}`});
+	sign(signOpts, { accessKeyId: `${credentials.accessKeyId}`.trim(), secretAccessKey: `${credentials.secretAccessKey}`.trim()});
 
 	const options: OptionsWithUri = {
 		headers: signOpts.headers,
diff --git a/packages/nodes-base/nodes/S3/GenericFunctions.ts b/packages/nodes-base/nodes/S3/GenericFunctions.ts
index 00f59b3f70..093b7cfb71 100644
--- a/packages/nodes-base/nodes/S3/GenericFunctions.ts
+++ b/packages/nodes-base/nodes/S3/GenericFunctions.ts
@@ -64,7 +64,7 @@ export async function s3ApiRequest(this: IHookFunctions | IExecuteFunctions | IL
 		body
 	};
 
-	sign(signOpts, { accessKeyId: `${credentials.accessKeyId}`, secretAccessKey: `${credentials.secretAccessKey}`});
+	sign(signOpts, { accessKeyId: `${credentials.accessKeyId}`.trim(), secretAccessKey: `${credentials.secretAccessKey}`.trim()});
 
 	const options: OptionsWithUri = {
 		headers: signOpts.headers,